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COrDIS Plus STR Amplification Kit


COrDIS Plus is a multiplex kit for amplification of 19 polymorphic STR-markers and amelogenin loci. The kit is intended to be used in human identification and biological relationship testing, cell line authentication, and analysis of biological chimerism after transplantation of human organs and tissues. The kit COrDIS Plus includes contemporaneously STR-markers from two systems of international standards of molecular genetic human identification: American CODIS and European ESS. This allows achieve extremely high informative research and compatibility of the obtained results with any existing forensic data bases.

The main features:

  • Contemporaneous analysis of 20 markers.
  • Compatibility with the genetic analyzers ABI 310/3130/3500, MegaBACE, NANOFOR  05.
  • Support of data analysis in GeneMapper Software, Genemarker, Genotyper, OSIRIS software.
  • The kit components are already dispensed into the tubes and lyophilized. Such a format allows to simplify labour, increase stability and reduce contamination risks. Transport and storage is carried out at room temperature. The shelf-life of the kit is 18 months.
  • The opportunity to carry out the direct analysis passing by the step of DNA purification. Lysis reagent COrDIS Sprint using for buccal swabs and Direct Activator reagent for blood samples by using direct PCR.
  • High resistance to the presence of PCR inhibitors (humic acids, hematin, indigo carmine).
  • The kit contains all the necessary consumables and reagents for the full cycle of research (PCR-tubes, 96-well microplate for capillary electrophoresis, DNA size standard and allelic ladder).


The kit includes the following STR-loci:

Locus Chromosome CODIS ESS
AmelogeninX Xp22.1-22.3 sex determination marker
AmelogeninY Yp11.2
D3S1358 3p21.31 + +
TH01 11p15.5 + +
D12S391 12p13.2   +
D1S1656 1q42   +
D10S1248 10q26.3   +
D22S1045 22q12.3   +
D2S441 2p14   +
D7S820 7q21.11 +  
D13S317 13q31.1 +  
FGA 4q31.3 + +
TPOX 2p25.3 +  
D18S51 18q21.33 + +
D16S539 16q24.1 +  
D8S1179 8q24.13 + +
CSF1PO 5q33.1 +  
D5S818 5q23.2 +  
VWA 12p13.31 + +
D21S11 21q21.1 + +
SE33 6q14 the most polymorphic marker




COrDIS Plus Allelic Ladder


Amplification of 1ng of human DNA (control 007) using COrDIS Plus kit


  DNA ammount (ng) Profile Reproducibility
1 1,0000 full reproducible
2 0,6667 full reproducible
3 0,4444 full reproducible
4 0,2963 full reproducible
5 0,1975 full reproducible
6 0,1317 full reproducible
7 0,0878 full reproducible
8 0,0585 full reproducible
9 0,0390 partial  
10 0,0260 partial  
11 0,0173 partial  
12 0,0116    
13 0,0077    

Serial Dilution of Genomic DNA for Sensitivity Studies


Amplification of 1ng of human DNA (control 007) using COrDIS Plus kit


Buccal epithelium samples was lysised using COrDIS Sprint reagent and amplified using COrDIS Plus kit.



  COrDIS Plus User Manual
  COrDIS Plus flyer
  Developmental validation of COrDIS Plus kit