Reagent kit for multiplex analysis of 15 microsatellite markers of 14 goat microsatellite markers.

The kit is intended for molecular genetic characterization of goats for the purpose of analyzing kinship and DNA individualization of animals based on multiplex PCR analysis of 14 loci, containing short tandem repeats (STR).

Markers make up a standard panel , recommended by the International Society of Animal Genetics (International Society of Animal Genetics - ISAG): INRA006, ILSTS87, ILSTS008, CSRD247, OarFCB20, ILSTS19, INRA063, SRCRSP8, MAF065, SRCRSP5, McM527, SRCRSP23, INRA005, INRA023.

The COrDIS Goat kit is a new generation multiplex test system, based on the unique technology of DNA amplification conditioning (COrDIS STRICT). The extremely wide analytical range of the COrDIS Goat kit allows you to conduct research without the stage of preliminary measurement and normalization of DNA concentration. The amplification conditioning technology provides an optimal level of the fluorescent signal, regardless of the amount of sample added to the reaction. Immediately after the DNA extraction stage, the maximum amount of the sample can be added to the amplification reaction, which will ensure high sensitivity of the analysis without the risk of "overloading" the amplification signal.

KEY FEATURES
  • Simultaneous analysis of 14 goat DNA individualizing markers
  • Markers, recommended by the International Society of Animal Genetics (International Society of Animal Genetics — ISAG)
  • Unique technology for normalizing the fluorescent signal of PCR products without measuring DNA concentration
  • High resistance to the presence of PCR inhibitors
  • Accelerated protocols for testing blood samples using the COrDIS Sprint reagent
TARGET MARKERS

REFERENCE SAMPLE

USER MANUAL

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