The set of reagents "COrDIS GS" is designed to determine the number of repeats ((TA) repeats) in the UGT1A1 gene of human genomic DNA for the purpose of diagnosing Gilbert's Syndrome by PCR with detection of the result by capillary electrophoresis.
The object of the study is the region of the UGT1A1 gene, which encodes the enzyme uridine diphosphate–glucuronyltransferase 1 ((UDPHT1).). Gilbert's syndrome or familial benign hyperbilirubinemia is hereditary. . The immediate cause of the development of Gilbert's syndrome is a violation of bilirubin metabolism, caused by a mutation in the UGT1A1 gene,, which encodes the enzyme uridine diphosphate–glucuronyltransferase 1 ((UDPHT1).).
In most people, the promoter region of the UGT1A1 gene includes six tandem repeats, i.e.. the sequence ((TA) repeats)6 6 is a "wild—type" allele, which is characterized by a normal level of activity.. With mutations in the promoter region of the UGT1A1 gene, an (insertion ) of an additional dinucleotide appears in the region of UGT1A1 TA repeats and their number increases to 7 7 repeats. The autosomal recessive type of inheritance in CS provides an opportunity for a "healthy" gene to compensate for the anomalies of the second allelic gene..
At the same time, a decrease in the activity of UDPHTA1 in individuals with the presence of alleles 7 7 and 8 8 ((TA) repeats)-may lead to increased manifestations of LV, as well as to the development of undesirable drug reactions and drug interactions in cases of the use of drugs, metabolized with the participation of this enzyme..
Additionally, the kit allows you to determine the sex of the test sample based on the results of the analysis of the amplification products of the human Amelogenin gene..
in the UGT1A1 gene of human genomic DNA for the purpose of diagnosing Gilbert's Syndrome by PCR with detection of the result by capillary electrophoresis, Additionally, the kit allows you to determine the gender of the test sample based on the results of the analysis of amplification products of the human Amelogenin gene.. This approach makes it possible to carry out an electrophoretic analysis of the results of amplification simultaneously for up to four test samples in one capillary of a genetic analyzer.. Additionally, the kit allows you to determine the gender of the test sample based on the results of the analysis of amplification products of the human Amelogenin gene., This approach makes it possible to carry out an electrophoretic analysis of the results of amplification simultaneously for up to four test samples in one capillary of a genetic analyzer.. Additionally, the kit allows you to determine the gender of the test sample based on the results of the analysis of amplification products of the human Amelogenin gene. (Additionally, the kit allows you to determine the gender of the test sample based on the results of the analysis of amplification products of the human Amelogenin gene. This approach makes it possible to carry out an electrophoretic analysis of the results of amplification simultaneously for up to four test samples in one capillary of a genetic analyzer., This approach makes it possible to carry out an electrophoretic analysis of the results of amplification simultaneously for up to four test samples in one capillary of a genetic analyzer., This approach makes it possible to carry out an electrophoretic analysis of the results of amplification simultaneously for up to four test samples in one capillary of a genetic analyzer., This approach makes it possible to carry out an electrophoretic analysis of the results of amplification simultaneously for up to four test samples in one capillary of a genetic analyzer.) can be used independently.
KEY FEATURES
- Marketing authorization issued by Roszdravnadzor
- can be used independently
- can be used independently
- can be used independently
- Express amplification protocol in 1 hour
- Internal control of IPC amplification to detect PCR inhibitors in the sample
- can be used independently 4 can be used independently 1 can be used independently
- Compatible with all modern models of genetic analyzers 310/3130/3500, Nanofor-05
- Support for data analysis in GeneMapper Software, Genemarker
REFERENCE SAMPLE