The COrDIS MSI reagent kit is designed to detect microsatellite instability (MSI) and impaired functioning of the human genomic DNA repair system, isolated from biomaterial in paraffin blocks (FFPE), by polymerase chain reaction followed by fragment analysis of amplification products by capillary electrophoresis.

Analysis of microsatellite instability is of great practical importance for the diagnosis and choice of treatment strategy for malignant colon tumors, including hereditary non-polypous colon cancer (lynch syndrome). The object of study in the analysis of microsatellite instability with the COrDIS MSI kit are five microsatellite markers (BAT-25, BAT-26, NR-21, NR-24 and NR-27). The studied markers are regions of mononucleotide repeats of genomic DNA, commonly found in the human genome in a homozygous state.

Additionally, the kit allows you to individualize the samples and confirm the origin of the biomaterial from a particular patient using polymorphic loci of human chromosomal DNA D12S391, CSF1PO, vWA, D21S11. This approach allows you to verify the origin of the test sample of biomaterial from a particular patient (in case of coincidence of the established allelic variants in the test material and the patient’s blood sample) and exclude the possibility of contamination of samples with extraneous biological material.

KEY FEATURES
  • Availability of registration certificate of Roszdravnadzor
  • Simultaneous Multiplex Analysis 5 mononucleotide markers
  • Identification of the origin of samples by 4 tetranucleotide repeat
  • Accelerated Amplification Protocol Over 1 o'clock
  • Amplification fluorescence detection
  • Compatible with all current ABI genetic analyzer models 310/3130/3500, Nanofor-05
  • Support for data analysis in GeneMapper Software, Genemarker
CHECK SAMPLE

PRINCIPLE OF ANALYSIS